Hi Art,
In a nutshell, yes. I understand that wild type GFP excites optimally at
405 with a shoulder at 488, the commonly used EGFP ("red-shifted") mutant/s
is/are mutated so that the 488 shoulder is better than 405. EYGFP has
further mutations that stretch its emission further into the yellow. There
are also UV-excitation enhanced versions (350-405 only with the 488
shoulder knocked out) and blue-emission enhanced ones. The nomenclature of
these things is a bit out of control, and it can be hard to work out which
one people are talking about when they want to use them. (Note that there
are also human-codon optimised versions which express better in mammalian
cells than those based on the wild type jellyfish sequence )
Clontech's web page seemed a good source of information last time I looked,
and they've got some sort of overview at:
http://www.clontech.com/clontech/Manuals/GFP/Intro.html
hope this helps
Ray
At 10:26 am -0500 9/2/99, Art Roberts wrote:
>Hi All,
>
>Does anyone know if GFP is well stimulated at 488 nm? Thanks.
>
>Art Roberts
>Dept. of Medicine
>Robert Wood Johnson Medical School
>
>email: robertar@umdnj.edu
>phone: 732-235-7790
>Fax: 732-235-7792
Ray Hicks
________________________________________________________________________
|University of Cambridge |Tel 01223 330149 |
|Department of Medicine |Fax 01223 336846 |
|Level 5, Addenbrookes Hospital |e-mail <rh208@cus.cam.ac.uk> |
|Hills Road Cambridge |Web http://facsmac.med.cam.ac.uk |
|CB2 |ftp server ftp://131.111.80.78 |
|UK | |
|_________________________________|_____________________________________|
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:53:06 EST