Hello, I've got an investigator who would like to look at GFP expression, BrDU uptake and cell cycle information simutaneously. Up to this point he's been measuring GFP with Hoechst staining for cell cycle with good success. Now, however, the denaturation step used in the BrDU protocol has added a considerable wrinkle to the process. We've suggested trying PI or 7AAD as an alternative to the Hoechst for cell cycle. Can anybody suggest a good starting point for a protocol to measure these parameters simultaneously? Any help would be appreciated. Thanks in advance, Holly Holly B. Lamb Flow Cytometry/Optical Morphology Core Facility 1414 Guggenheim Bldg Mayo Foundation 200 1st St. S.W. Rochester, MN 55905 lamb.holly@mayo.edu pager 127-5198 (507)284-4241 fax (507)284-1767
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