PBMC and Confocal Microscopy

From: Richard Meister (meister.1@osu.edu)
Date: Tue Feb 09 1999 - 13:57:15 EST


Hello, all:

I have a student who wants to do confocal laser scanning microscopy on
peripheral blood cells.  He wants to do intracellular staining, and, since
the cells are non-adherent, needs to know the best method for immobilizing
(fixing) the cells onto a glass slide for confocal microscopy.  Any ideas
would be greatly appreciated.

Thanks in advance,
Richard Meister



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