Dear Kathy, You may want to try DAKO Ki-67 (Code No. F0053) FITC conjugated antibody for paraffin-embedded samples. The antibody is directed against a nuclear antigen and requires permeabilization first (DAKO IntraStain). Our package insert describes the procedure in detail. Please feel free to call me with any questions or visit our website at www.dakousa.com Respectfully, Karen Semiao Product Manager Flow Cytometry DAKO Corporation 800-400-3256 ext 5480 -----Original Message----- From: Altig,Kathy [mailto:kaltig@swmedctr.com] Sent: Monday, February 08, 1999 11:32 AM To: cyto-inbox Subject: Ki-67 We are planning to perform Ki-67 on paraffin preserved tissue by flow. This will replace the ploidy analysis, as it seems to be out of favor. I am trying to find the best or most practical method and antibody. Suggestions? Thank you, Kathy Altig SW Washington Med. Ctr.
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