anti human beta actin and anti MDC antibodies

From: paukovic@burnet.edu.au
Date: Sun Feb 07 1999 - 23:15:46 EST


deer fellow flow fiddlers,

my main question: Is there a manufacturer of anti human beta actin antibody
(+/- fluorochrome) for flow use...

other associated questions:

is there anyone out there whom quantitates beta actin levels
(intracellularly) using flow?? is there a protocol??

does anyone out there know if levels of beta actin remain constant in
stimulated (eg PHA @ 2.5ug/ml) lymphocytes or do they increase with the size
of the cell (eg is there more beta actin in blasts???)

and lastly is there an antibody against MDC (mono/macrophage derived
chemokine) for flow use or is there anyone working on it???

fank you all for your time and suggestion in advance
cheers
Geza Paukovics - Flow Laboratory - AIDS Pathogenesis Research Unit
Macfarlane Burnet Centre for Medical Research
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