A major problem probably is the actual number of binding sites for your staining reagent on each phage particle. This might be below the detection limit of your machine. A second problem might be the staining reagent. If it is not an antibody, you may want to check if the affinity is sufficiently high (or the koff sufficiently low) to prevent detachment during the test. Saverio Alberti Head, Unit of Experimental Oncology Department of Cell Biology and Oncology Consorzio Mario Negri Sud 66030 Santa Maria Imbaro (Chieti), Italy Phone: (39) 872-570.293 FAX: (39) 872-578.240 E-mail: alberti@cmns.mnegri.it On Wed, 3 Feb 1999, Luca Battistini & Giovanna Borsellino wrote: > > > Dear cytometrists, > > We are trying to sort filamentous phage on a Cytomation MoFlo. Phages are > pretty tiny, being around 1 micron in length and less than 0.07 micron in > width. We can actually see it and sort it, but so far have been unable to > stain it. > > Any ideas will be greatly appreciated! > > Cheers > > Giovanna Borsellino > IRCCS S.Lucia > Rome, Italy > >
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