modulation of CD4

From: Kathleen Schell (kschell@facstaff.wisc.edu)
Date: Mon Feb 01 1999 - 14:29:39 EST


Dear Flowers,
     We have an investigator who is studying cytokine production in
response to mitogenic stimulus.  We observe a down regulation of the CD4
molecule ( in surface staining) by 24 hours that renders this population
unidentifiable.  They are looking at intracellular cytokines, and surface
molecules are stained first,  cells are fixed, permeabilzed, and then
stained for cytokines.  Has anyone seen this?  We have repeatedly seen a
decrease in the fluorescence of CD3 after PHA stimulation, but never to the
point where the population can no longer be seen--this has been reported in
the literature.  Is there another way to identify the CD4 population using
this kind of staining protocol?  Cells not stimulated have good surface
expression of CD4.
Thanks in advance.
Kathy

--------------------------------------------------------------
Kathy Schell
Supervisor, UWCCC Flow Cytometry Facility
600 Highland Ave.  K4/535
Madison, WI 53792
Voice:  608-263-0313
e-mail:  kschell@facstaff.wisc.edu



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