Just a quick caveat about the article and technique: They are using GFP that
localizes to the ER, not your regular old cytoplasmic GFP.
-Jim
At 12:26 PM 1/29/99 +0000, Ann Atzberger wrote:
>
>Hallo everybody,
>
>In the journal Biotechniques 26:102-106 there's a report on Cell Cycle
analysis
>in transfected cells, using Digitonin treatment of cells instead of ethanol
>fixation. Digitonin preserves the GFP fluorescence. They show CV's of
>average 4% for U2OS and NIH 3T3 cells.
>
>Authors:Dimitri G. Pestov, Marina Polonskaia and Lester F. Lau.
>University of Illinois College of Medicine.
>
>
>
>If any of you out there are interested in this and can't get the journal,
>let me know and I will send the protocol.
>
>
>Ann Atzberger.
>
>
>
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