>We have been analyzing apoptosis cell death in a human colon >adenocarcinoma cell line, HT29. We have obtained very nice and clear >results, but we usually detect a 3 to 5% annexin (-)/ PI (+) >population. >We can observe the same result with other cell lines and different >stimuli, and I have been asked to explain what is happening >there. > Many articles don't make any difference between PI (+) cells, beeing >annexin (+) or (-). Could someone suggest how to describe these cells ? > >Thank you very much. > >Mireia We have also seen free nuclei in the PI-positive/Annexin V-negative quadrant. Eric /\/\/\_ Eric Van Buren, aa9080@wayne.edu \ \ \ Karmanos Cancer Institute and Immunology & Microbiology \_^_/ Wayne State University, Detroit, Michigan, USA
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