In my experience, PI =ve/ annexin -ve cells have probably died by necrosis. I have a data file (to be included in the second edition of my CD-ROM, when I finally complete it) of a PI/annexin stain of cultured human leucocytes. The granulocytes are dying by apoptosis and are PI +ve/annexin -ve. A population of lymphocytes are PI +ve and annexin -ve. I have seen other examples. Although the dogma says that annexin will enter a necrotic cell and bind to PS on the internal face of the plasma membrane, I have not seen anyexperimental evidence (perhaps because, as yet, I have not looked very hard). The little evidence I have suggestes that, in some cells at least, this does not happen. Michael Ormerod 34 Wray Park Road Reigate RH2 ODE Telephone: voice & FAX: (0)1737 241726 Web site: http://ourworld.compuserve.com/homepages/Michael_Ormerod
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