Hi Vanessa. I've run mouse LGM cells with strepavidin-RED670, result: high background, with Southern BioTech's Neutralite Avidin, the background was reduced to a level which was acceptable in the assay I was using. I posted a reply to a similar question earlier this year which should be in the archive. Arnold >Date: Wed, 27 Jan 1999 16:45:59 -0500 (EST) >From: Vanessa Ruta <vruta@hejira.hunter.cuny.edu> >To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu> >Subject: bone marrow and strepavdin >MIME-Version: 1.0 > > >Forgive my ignorance. >We are finding that there is a high level of background fluorescence >staining mouse bone marrow with a biotinylated >antibody-fluorescent strepavdin system. >Has anyone tried neutorlite avdin (Southern BioTech) a deglycosolated >version on bone marrow? Or does anyone have any suggestions? >Thanks >Vanessa Ruta -------------------------------- Arnold R Pizzey Department of Haematology UCL Med. School 98 Chenies Mews London WC1E 6HX, England Tel 44-171-209-6234 FAX 44-171-209-6222 a.pizzey@ucl.ac.uk -------------------------------
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