This message brings up a point that I'd like to see addressed by the experts. In my hands, FITC (as well as PE, PerCP, APC, PI, 7-AAD, and ToPro-3) is very stable, and no precautions need to be taken while staining or running samples, as no degradation has ever been seen. Every clinical and research lab I've been in stains samples on the bench top and takes no special precautions to avoid any forms of light. But some researchers here do things like staining in the dark, encasing samples in aluminum foil, and using red filters on the room lights in the flow lab for fear of fluorochrome degradation. Based on my experience, I'm not sure why they fear the light to this extent. I've seen this happen using the fluorescent scope, but I think those are usually fluors like Cy2 and Cy3. Are some derivations of fluorescein more photolabile than others, or am I ruining my samples. thanks for any news and views. Keith Bahjat kbahjat@ufl.edu joan Kalnitsky wrote: > Does anyone know the quenching time of PI? We have someone who wants to > stain with PI and is concerned about whether it will fade out like FITC > would. Also, how about PE. Is it as light sensitive as FITC. Any and all > suggstions would be helpful. If anyone has stained with PI and has a > protocol, we would love to see it. > Thanks in advance. > Joan K > > > > Flow Cytometry Lab Supervisor > VMRCVM > (540) 231-4115 > FAX 540-231-7367 > jkalnits@vt.edu > > "It is better to serve than to receive." > B. Borg
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