Live DNA staining

From: KMCDONALD (KMCDONALD@MICROIMM.MCGILL.CA)
Date: Sun Jan 24 1999 - 22:11:49 EST


Hi flowers,
            Does anyone know a method of staining cells for DNA cell
cycle analysis with the intension of sorting the various phases and
culturing them. I can only think of using Hoechst staining (sterile) and
then sorting, however I've heard that Hoechst can be toxic.
Thanks for your responses in advance...Ken McDonald



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