Dear Flow Ers, I am working with punch of smart people who has no flow backgrounds. They are experts in their fields. we manufacture activated lymphocytes to implant into various tumors. FDA needs validation for all my test methods. 1. CD 69 to measure early activation T3+ cells 2. RNA and DNA stainig by AO ( acridine orange) They want me to show a)precision b)repeatability c) robustness d) accuracy. When I talk about gating: i say gate on lymphocyte. They ask me to prove that the cells in the gate are lymphocyte. Please any body save me. I appreciate all your help and any help at all. I promise i wiil post the out come. Thanks for saving my soul in advance. Mathi Cytometry Meyer Pharmaceuticals mathi@meyerpharm.com
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