I haven't done it, but you should be able to fake a compensation matrix that will add FL1 + FL2, then derive a ratio using that new one. We considered and dismissed the generalized parameter calculator from FlowJo because no one had (previously) expressed the need. If your colleague can explain why that helps, and it makes sense for others, then we'll support it. There is a problem that FCS files don't have negative numbers in them, so a general algebraic parser wont work. Adam On Wed, 13 Jan 1999, Joseph Webster wrote: > > Hi All, > A slightly curly request.... :~? > > A colleague wants the ratio of (FL2 / (FL1 + FL2)) from many flow readings. > (He explained why, but I didn't understand...) > > He wants this from many datafiles, so batch processing probably.... > He wants to export this into some other program such as Excel..... > > Several computer packages can produce simple ratios of one parameter > over another, but someone is always wanting more... > > Any ideas? > > Another challenge for Ray Hicks, Adam Treister, Joe Trotter Et Al ;~) > How about the ability to enter a user-defined function or formula to be > applied to the data? > > (The "massaging" possibilities are amazing!-) > > Thanks, Joseph. > > -- > Joseph Webster > Flow Cytometry Facility > Centenary Institute >
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