Dear Heidi, we have used 7-AAD for live/dead discrimination in different cell lines with good success. Because of its longer emission wavelengt, 7-AAD is less critical when used in combination with FITC and PE than PI. In some cases we have observed a negligible uptake of 7-AAD (and PI) into living cells after prolonged incubation periods. See Cytometry 13: 204-208 (1992). Hope this helps Georg ------------------------------------------------------------------------ H.G. Kreysch Merck KGaA Biomed Fo/IMO - A25/410 Tel.: (49)6151 - 72 7323 Frankfurter Str. 250 FAX: (49)6151 - 72 7398 D-64271 Darmstadt E-Mail: kreysch@merck.de
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