TIM: ABOUT THE ARTICLE OC CYTOMETRY 33:385-393,1998, I THINK THAT IS HEAVY, HARD AND EXCELLENT. THERE SOME ASPECTS THAT MAY BE HELP YOU: 1) IN THE "ANALYTICAL PROCEDURES", THA AUTHORS DESCRIBE VERY WELL (BUT ARIDLY), THE ORIGIN OF DIFFERENT FITTING`S MODELS. A VERY GOOD DESCRIPTION OF THAT, YOU CAN FIND IN REFFERENCE NUMBER 40 OF THE SAME ARTICLE. 2) THE SPF CALCULATIONS METHODS, IN MY OPINION, COMBINED TWO DIFFERENT MATEMATICAL MODELS (BROADENED TRAPEZOID IN MODFIT AND ZERO-ORDER BROADENED POLYNOMIAL IN MULTICYCLE). ALSO I THINK THAT IS IMPORTANT THE G2M FRACTION. THE PROLIFERATION FRACTION (S-PHASE + G2M) MAY BE A GOOD INDICADOR OF CYCLING CELLS. 3) FINALLY THE AUTHORS, WRITE THAT FOR THE ARTICLE. THE DEBRIS AND AGGREGATES "DOES NOT SEEM TO HAVE ANY ADVANTAGE" IN FITTING. IF YOU USE FRESH TISSUE, ALWAYS THE AGGREGATES WILL PRESENT PROBLEMS (IS DIFFICULT ELIMINATE DOBLETS, AND THE DEBRIS CAN AGGREGATE TOO). IF YOU USE FIXED TISSUE (PARAFFIN), YOU MUST COMPENSATE DEBRIS, EXCEPT IF THE EVENTS ARE GATED. IN THE SAME NUMBER OF CYTOMETRY, THERE ARE OTHER ARTICLE OF THE SAME AUTHORS (PAGE 487-491) AND IS VERY INTERESTING. IN MY EXPERIENCE, THE CORRECT FITTING OF A DNA HISTOGRAM IS DIFFICULT, AND ALWAYS MUST BE USE "THE SAME MODEL" FOR YOUR EXPERIENCE WITH A DETERMINATED TISSUE. IN EXAMPLE , I USE THE MODFIT LT AND THE BROADENED TRAPEZOID IN FRESH COLON AND STOMACH (I DEFINED THE CUTOFF).IN PARAFFIN I PREFERED USE BROADENED RECTANGLE. ADIOS JUAN LUIS CASTILLO NAVARRETE LABORATORIO DE CITOMETRIA DE FLUJO HOSPITAL DEL TRABAJADOR, CONCEPCION. CARDENIO AVELLO 36 CONCEPCION CHILE PHONE: 56-41-201722 FAX: 56-41-201708 EMAIL: axelyoyi@entelchile.net > > Dear all DNA analysis people, > A recent article was published in Cytometry on S phase analysis and > prognosis in breast cancer. The title of the article and reference are > listed below: > > Different calculation methods for flow cytometric S-phase fraction: > prognostic implications in breast cancer? > Bo Baldetorp et al Cytometry 33 385-393,1998 ( Dec 1 issue) > > I would be interested in hearing comments on this paper. I will try to > summarize the comments after a two week period. I believe that this paper > has much "food for thought". > > Tim Kute > Dr. Timothy Kute Telephone # = 336-716-2950 > Associate Professor in Pathology email = tkute@bgsm.edu > Wake Forest University School of Medicine > Medical Center Blvd > Winston-Salem, NC 27157-1072
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