Hi, I stain mouse splenic lymphocytes, and I use a product called Fc Block from PharMingen (catalogue number 01241D, clone 2.4G2) It binds to CD16 and CD32 (Fc gamma receptors III and II). You might also try to titrate your antibodies to find the optimal concentration giving the best signal with the lowest amount of background staining. Julia >Hi collegues, >please accept me as a new member of the FACS-User-Group. >My first question: Has anyone experience in staining mouse splenocytes? I >have a problem with the high unspecific binding of several antibodies. Does >anybody know a method how to overcome this Problem. > >Thanks in advance > >Alwina ---------------------------------------------------------------------------- -------------- Julia Philips PhD student Institute of Dental Research 2 Chalmers St Surry Hills NSW 2010 Australia ph: xx-61-2-9293 3358 fax: xx-61-2-9293 3368
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