Olindo ---------- From: Dr. Alwina Bittner[SMTP:bittnera@mailer.uni-marburg.de] Sent: Sexta-feira, 8 de Janeiro de 1999 08:23 To: cyto-inbox Subject: new member - unspecific antibody binding to mouse splenocytes Hi collegues, please accept me as a new member of the FACS-User-Group. My first question: Has anyone experience in staining mouse splenocytes? I have a problem with the high unspecific binding of several antibodies. Does anybody know a method how to overcome this Problem. Thanks in advance I work with mouse spleenocytes, PBL, LNL and BM. I always had unspecific binding before I start to blok this undesired reactions with PBS 3% FBS and also with 1% of serum form the source of my monoclonal. Good luck. Alwina Dr. Alwina Bittner MZ Nervenheilkunde - Neurologische Klinik mit Poliklinik Neuroimmunologisches Labor Rudolf-Bultmann-Str.8, 35039 Marburg, Germany, Fax +49 6421 28 7055, Phone +49 6421 28 5480
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