>To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu>
>From: "Douglas S. Smoot" <dsmoot@med.navy.mil>
>Subject: Platelets
>Today I was asked about running platelets on my flow cytometer. I've never
>run them, so are there any special tricks that I should be aware of? I have
>a Coulter Elite, if that makes a difference. Thank you.
>Doug
>{-----------------------------------------------------------------------}
>Douglas Smoot
>Immune Cell Biology Program
>Naval Medical Research Center
>8901 Wisconsin Avenue
>Bethesda, MD 20889-5607
>voice: 301-295-6272
>fax: 301-295-0376
Hi Doug.
I ran some Thiazole orange stained platelets on our ELITE a few months ago
and I don't recall any special problems, I used FS log/PMT1log trigering
off of FSlog, a discriminator on the FS was used to ignore noise signals.
If you need any further help e-mail me direct and I'll send you the
protocol file I used
Regards
Arnold
--------------------------------
Arnold R Pizzey
Department of Haematology
UCL Med. School
98 Chenies Mews
London WC1E 6HX, England
Tel 44-171-209-6234
FAX 44-171-209-6222
a.pizzey@ucl.ac.uk
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