Colleagues ... I need to start to measure expression levels of certain epitopes on human leucocytes and will be using specific mouse IgG1 MAbs. I guess these are used at saturating concentrations, followed by comparison of intensities with fluorochrome-conjugated anti-mouse-IgG between stained leucocytes and mouse IgG-bearing calibrator beads. I think my anti-mouse-IgG conjugate is at last OK, thanks to some helpful suggestions here. Can anyone suggest sources of reliable calibrator beads and I would welcome any comments on techniques and pitfalls - also a few good quotable references. Robin Barclay SNBTS, Edinburgh
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