Good luck
Olindo
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From: Douglas S. Smoot[SMTP:dsmoot@med.navy.mil]
Sent: Segunda-feira, 4 de Janeiro de 1999 19:10
To: cyto-inbox
Subject: Platelets
Today I was asked about running platelets on my flow cytometer. I've never
run them, so are there any special tricks that I should be aware of? I have
a Coulter Elite, if that makes a difference. Thank you.
Doug
{-----------------------------------------------------------------------}
Douglas Smoot
Immune Cell Biology Program
Naval MedicalI have worke with platelets since last August and there are no big deal
on perforning the method. I have used the one suggested by BD from their
lab methods book. The only thing I would advice you is if you will work
with not fixed platelets it is good to use PBS suplemmented with EDTA
0,009-0,0013M to avoid platelet activation. I have a FACScalibur and
the acquisiton is made using log scale for both FSC and SSC. I have no
experience with coulter. Should be something similar, doesn't it?
Research Center
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Bethesda, MD 20889-5607
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fax: 301-295-0376
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