We use a Coulter Elite for platlet activation studies on a regular basis
without much difficulty. We use a pan-platelet marker to help pull them
out of debris, but I have gated on light scatter with them in the past.
You shouldn't have much difficulty (famous last words)
At 04:10 PM 04/01/1999 -0500, Douglas S. Smoot wrote:
>
>Today I was asked about running platelets on my flow cytometer. I've never
>run them, so are there any special tricks that I should be aware of? I have
>a Coulter Elite, if that makes a difference. Thank you.
>
>Doug
>{-----------------------------------------------------------------------}
>Douglas Smoot
>Immune Cell Biology Program
>Naval Medical Research Center
>8901 Wisconsin Avenue
>Bethesda, MD 20889-5607
>voice: 301-295-6272
>fax: 301-295-0376
>
>
Derek Schulze
Queens University, Cancer Research Laboratories
Flow Cytometry and Confocal Microscopy Services
Kingston, Ontario, Canada
flow@post.queensu.ca
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