Dear flow friend we have a 38 y/o woman whose diagnosis is challenging us. She was admitted in our hospital for deep anemia (6 gr/dl) and low platelets count (15000 mmc) discovered during a routine blood test after an antibiotic resistant fever. Clinically the patient has and enlarged spleen (4 cm below the costal arch), an enlarged mediastinum caused by the presence of several lymphonodes, and a pleural effusion. The blasts looks like small undifferentiated cells and we have found several figures of emophagocitosis. The immunophenotype of the cells is: CD7 positive (strong) CD5 positive (dim) CD45 positive (dim) Gly-A positive (dim in 35% of the cell, but this monoclonal sometimes give us questionable results!) all the other markers we have tested are negative including: CD2, cCD3, sCD3, CD4, CD8, CD9, CD10, CD11c, CD13, CD14, CD19, CD20, cCD22, sCD22, CD33, CD34, CD36, CD41, CD42, CD117, DR. Tdt is also negative as well as mieloperroxidase with the exxception of 1% of the CD7 cells which are double positive for CD7 and MPO. Tomorrow we will stain our cells with CD1, MAC and few other MoAbs which are positive on hystiocytes and macrophages but we are anxious to have some clues from the discussion group. Cytogenetic is on the way. Thank you for your advices. ------------------------------------------ Ugo Consoli, M.D. Institute of Hematology University of Catania, Italy Fax ++39-95-7311510 Tel. ++39-95-7435917 - 7435922
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:51:44 EST