Hello to all,
One of the PI's here at the University of Miami has a question. Here
is what she wants to do:
Immunize a mouse with an antigen, wait 10-14 days, harvest lymph
nodes/spleen, do frozen sections, stain those frozen sections with
antibodies specific for mouse IL-4 or IFN-gamma, and detect cytokine
producing cells by immunohistochemistry or fluorescent microscopy.
Has this been done? Actually, she wants to use confocal but doesn't
think many people have access to confocal so they might have done it
by conventional fluorescent microscopy.
Any help would be appreciated.
Thank you
Jim Phillips
Please make all responses to
Dr. Rebecca Adkins
University of Miami School of Medicine
Phone 305-243-5561
Fax 305-243-4086
e-mail radkins@mednet.med.miami.edu
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