We are about to embark an a project that involves the detection and hopefully the sorting of human dendritic cells growing in human cancellous bone fragments implanted into scid mice (Blood 88:1973-1982, 1996). To begin with we will try to identify these cells in normal human marrow suspensions and if this is successful, move forward with the scid mouse experiments. One potential composite phenotype that we thought might be useful is: CD14-negative/CD1a-positive/CD83-positive. What is the status of pan-CD45 antigen expression on dendritic cells? What about CD45-negative/CD1a-positive/CD83-positive? Any advice/better ideas regarding these issues will be gratefully received. Rob Sutherland Oncology Research The Toronto Hospital
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