Dear John
You just have hit the big rock in the road. You can see the DC with veils
and dendrites in the lower part of the granulocytes area. However, if you
have them treated with enzymes, they will be more o less round and close
to the monocytes area. A few days ago, we sorted human DC after culturing
with IL-4 and GM-CSF from the lower granulocyte area and it worked fine.
Good luck
Rafael
>Dear All,
>
>We are trying to characterise dendritic cells in stimulated cultures of
>PBMCs. We have a panel of potential markers of dendritic cells but are
>having problems finding their characteristic scatter patterns for gating
>purposes. Back gating gives no clear area. Can anyone help? Where do these
>cells lie on a normal FWD vs SSC plot of "whole blood" - in the
>LGL/monocyte area, or towards the neutrophils?
>
>Many thanks, John.
>
> Dr John Lawry,
>Institute for Cancer Studies, University Medical School, Beech Hill Road,
>Sheffield. S10 2RX.
>Tel:0114-271-3023 (lab), 0114-271-3774 (office)
>Fax:0114-271-3515
>Email: J.Lawry@sheffield.ac.uk
>Web page: http://www.shef.ac.uk/uni/academic/A-C/csi/Staff/John/JL.html
\|/
(o o)
________________________________oOo__(_)__oOo_________________________________
___/\_ | Rafael Nunez mailto:rafaeln@vetvir.unizh.ch
/ o \/| | University Inst.for Virology http://www.vetvir.unizh.ch/
/ _| | Winterthurerstr. 266a Telephone: (+41) 1 6358710
/_/\__/-\/ | 8057 Zurich SWITZERLAND Faximile : (+41) 1 6358911
______________________________________________________________________________
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