For those of you are interested. Thanks to those that responded Pb > ---------- > From: Lynn Dustin[SMTP:dustinlb@SLU.EDU] > Sent: Monday, January 26, 1998 11:52 AM > To: Barren, Phil; cytometry@flowcyt.cyto.purdue.edu > Subject: Re: CD2 Receptor Number on Human Lymphocytes and or > Jurkat cells > > I am forwarding the response below from Michael Dustin. Hope it's > helpful. > Lynn Dustin,Ph.D. > Assistant Professor > Molecular Microbiology and Immunology > St. Louis University > > >CD2 on Jurkat is variable with anywhere from 80,000-200,000 > sites/cell > >depending upon the clone. The ATCC cells are around 80,000 by our > >measurements. The average density of CD2 on resting peripheral blood > T > >cells is 50,000 per cell. Since there are two populations that > differ by > >2.5 fold the actual number on any single cells should be ~30,000 or > 70,000 > >depending on whether they are memory of naive phenotype. > > >Michael L. Dustin, Ph.D. > >Assistant Professor of Pathology > >Washington Unversity School of Medicine > >Department of Pathology > >Box 8118 > >660 S. Euclid Ave > >St. Louis, MO 63110 > > > > >>FLOWers > >> > >>Does anyone happen to know the CD2 receptor number on human > lymphocytes > >>or on Jurkat cells?? > >> > >>Thank you in advance for your help > >> > >>Philip Barren > > > >
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