PCNA staining

From: Altterrain (Altterrain@aol.com)
Date: Tue Jan 27 1998 - 14:43:50 EST

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hi all,

	i've been attempting anti-PCNA staining with coulter mAb19F4 FITC labelled in
combination with PE and PerCP Abs (CD33 or 38 and CD34). samples were analyzed
on a FACScan. i have tried the fix and perm reagent protocol provided by
coulter and caltag's fix and perm kit. results are less than spectacular on my
target cells, cultured CD34+ human cord blood cells. channel seperation of
PCNA pos./neg. (high/low?) populations is poor at best. are there any tricks
to doing this or is this the best i can hope for? thanks in advance for your
help.

-brian white
human genome sciences
rockville, md
brian_white@hgsi.com
301-309-8504 x2409

Next message: Noelle Williams: "murine CD2 antibodies"
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