Re: CD2 Receptor Number on Human Lymphocytes and or Jurkat cells

From: Lynn Dustin (dustinlb@SLU.EDU)
Date: Mon Jan 26 1998 - 11:52:26 EST


I am forwarding the response below from Michael Dustin.  Hope it's helpful.
Lynn Dustin,Ph.D.
Assistant Professor
Molecular Microbiology and Immunology
St. Louis University

>CD2 on Jurkat is variable with anywhere from 80,000-200,000 sites/cell
>depending upon the clone.  The ATCC cells are around 80,000 by our
>measurements.  The average density of CD2 on resting peripheral blood T
>cells is 50,000 per cell.  Since there are two populations that differ by
>2.5 fold the actual number on any single cells should be ~30,000 or 70,000
>depending on whether they are memory of naive phenotype.

>Michael L. Dustin, Ph.D.
>Assistant Professor of Pathology
>Washington Unversity School of Medicine
>Department of Pathology
>Box 8118
>660 S. Euclid Ave
>St. Louis, MO 63110



>>FLOWers
>>
>>Does anyone happen to know the CD2 receptor number on human lymphocytes
>>or on Jurkat cells??
>>
>>Thank you in advance for your help
>>
>>Philip Barren
>



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