deer fellow flow sufferers, I pose 3 questions, totally irrelevant to one another....I hope someone out there is listening...Fank you all for your time and answers in advance... 1. Recently I was asked if it possible to sort intracellular particles, eg. mitochondria etc.. Has anyone done this..how good are the yields? is there a protocol which may be employed or are there any helpful references...I have a dual laser FACStar Plus. There are so many questions....non-specific staining? Do I have to change the nozzle size (currently 75um), do I have to alter the drop drive frequency and to what optimal frequency? are there alternate methods other than flow? will I be battling with high abort rates? 2. Laser question...is anyone selling or know of a source of an air cooled He-Ne or Argon -ion laser emitting at ~600nm? I know Coherent sell them, but prefer maybe alternate prices??? 3. beads...I am looking for a source of Fc coated beads ~2um to be used in a phagocytic assay... I asked more questions than 3 didn't I? hope they weren't too inane... ekat erac lla Geza Paukovics - Flow Laboratory - AIDS Pathogenesis Research Unit Macfarlane Burnet Centre for Medical Research .***. .***. .** PO Box 254 _--_|\ * | | | * * | | Fairfield, VIC 3078 / \ * * | | | * * | | | AUSTRALIA. \_.--._/ * * | | | * | | | * ph. (+61 3) 9282 2132, O '***' '***' FAX (+61 3) 9282 2100
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