Hello Jane our laboratory in collaboration with Pharmingen/BD has developed a "clinical" procedure for the diagnosis or screening of possible XHIM patients. O'Gorman, et. al. Development of a rapid whole blood flow cytometry procedure for the diagnosis of x-linked hyper IgM syndrome patients and carriers. clin. immunol. immunopath. Vol.85 (2); 172-181, November 1997. the method is facile, can be completed during a work shift (<6hrs), is done using 3 colours on whole blood (heparin only) that can be held overnight, requires a little gating strategy but is easily interpreted after reading the manuscript if you have any questions about our experience with it or the procedure itself i'll be happy to help. Dr. MRG O'Gorman Assis. Prof. Pediatrics Northwestern University Medical School Director Diagnostic Immunology and Flow Cytometry Laboratories Children's Memorial Hospital, Chicago IL
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