Hello Dominique, There is a considerable learning curve on the BD turbo sort option and running a sort at high speed is definitely more demanding than at low speed. It takes quite some time (considerably more than a month) and practice to reach a really relaxed comfort level with the upgrade. The main thing is to get that drop delay to be stable -- without a stable drop delay your purity and recovery will always be terrible. Tricks that worked for us included moving the sterilizing filter closer to the flow cell and installing a fan to keep the electronic boards cool (we have a FACStar, not a Vantage). Glenn Paradis at MIT has compiled a list of TSO users -- perhaps he will let you have the list so that you can get their advice on specific questions. The BD option for high speed sorting definitely works and is definitely worth the time and frustration of getting used to the system. Alice Alice L. Givan Englert Cell Analysis Laboratory Dartmouth Medical School Lebanon, New Hampshire NH 03756 USA tel 603-650-7661 fax 603-650-6130 e-mail givan@dartmouth.edu
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