Hello, Since I worked in the lab that discovered PAC-1, I want to clear up some of the incorrect information about this antibody. PAC-1 is an antibody that binds only to the activated form of the platelet glycoprotein IIb-IIIa complex. It recognizes an epitope on the IIb-IIIa complex critical for fibrinogen binding. Therefore PAC-1 and fibrinogen inhibit each other's binding in a competive manner. If you are using a whole blood assay the blood should be diluted 1:10 to eliminate this effect. Also, the tetrapeptide, RGDS, competitively inhibits PAC-1 binding. Since PAC-1 is an antibody that recognizes only activated platelets, care must be taken in handlimg the platelets (ie. washed platelets). Some useful references are: 1. Shattil,S, et al. Blood: vol 70: 307-315, 1987 2. Abrams, C., et al. Blood: vol 75: 128-138, 1990 3. Shattil,S., et al. JBC: vol 260:11107-11114, 1985 I hope this information helps people using the PAC-1 antibody. Michael Cunningham
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