Me again! I need to work up calcium measurements on the FACStar Plus, and exploring the options. Since we have the right laser and mirrors, I wish to run the primary laser in multi line UV+VIS, so I can get the usual scatter along with calcium ratio in FL1 & FL2. We could also use the dye laser for other markers at the same time. While I'm waiting for the optical filters to arrive:- Has anyone tried this? Are there glaring reasons why it should not work? Any ideas for measuring the power of 488 & UV separately in multi line? (yes, I have a power meter) I know there are many people using other laser layouts, but we only have a two-laser bench and I REALLY don't want to move the dye laser in and out of position between experiments. Many Thanks, Joseph. -- Joseph Webster Flow Cytometry Facility Centenary Institute
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