Hello, everyone: One of our graduate students wants to undertake a project involving mast cells (in general, and canine mast cells in particular) and flow cytometry. A few questions: 1. Are there any mast cell lines available, either human, canine, etc.? 2. What would be the best way to isolate mast cells from solid tissue? (I imagine that some isolation protocols could cause the mast cells to degranulate and make identification more difficult.) 3. Do mast cells circulate in the blood as such, or as a precursor cell type? Can they be isolated from the blood? 4. What is the best way to identify mast cells by flow cytometry? Any information or references will be greatly appreciated. Thanks in advance.
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