The problem with supravital cell staining with pyronine Y is that at relatively low concentration (below 5 uM) the dye, similar as rhodamine 123, is localized in mitochondria, photosensitizes cells and binds minimally to RNA. At that concentration it arrests cells in G1. At concentration above 5 uM it also binds to RNA and then arrests cells in S (Cancer Res., 46: 5760, 1986). Pyronine Y can be used as a probe of RNA after cell fixation (Cytometry, 8: 138, 1987). Zbigniew Darzynkiewicz
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