Re: Apoptosis by flow

From: C. Kevin Becker (ckb@phnxflow.com)
Date: Sun Dec 21 1997 - 00:47:27 EST


-----Original Message-----
From: robert ashcroft <cytomat@netcore.com.au>
To: cyto-inbox
Date: Saturday, December 20, 1997 3:22 PM
Subject: RE: Apoptosis by flow


>
>-- On Wed, 17 Dec 1997 21:33:58 -0500 (EST)  Bob Donahoe
><rdonaho@emory.edu> wrote:
>
>>
>>    No surprises here, I'm lost and confused.  What is the best way to
>>analyze apoptosis in circulating leukocytes by cytofluorometry, especially
>>in regard to AIDS viral infection?  Thanks in advance and happy holidays.
>
I say, in regards to anything infected with AIDS, even if it isn't in the
X-mas spirit, kill the suckers before you put them on the flow cytometer!!!!
Therefore, you will want to use the TUNEL assay to measure apoptosis in
these dead suckers.  Who makes the most sensitive, least expensive, most
complete (including positive control cells) TUNEL kit?  Phoenix Flow
Systems.  Check out our APO-BRDU kit on our web page at www.phnxflow.com.

Merry X-mas to every one

   ______   ______  _______
 /_____/  /_____   /______   C. Kevin Becker
/            /             ______/ Phoenix Flow Systems, Inc.
11575 Sorrento Vlly. Rd. #208, San Diego, CA 92121 USA
(619) 453-5095 FAX (619) 259-5268 ckb@phnxflow.com



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