Simon Monard wrote: > >You don't say if its human material you are staining, its very common in >Macaques to see double positive cells, sometimes accounting for a third or >more of total lymphocytes. As you work for the army I suspect your subjects >are mostly human , I see it at very low frequency in humans maybe 1 or 2 >percent at most, I put it down to artifact, doublets or something. > >What species and what frequency? If the frequency is quite high and its >human sounds like one for our hematology experts out there. > >Simon Monard >Aaron Diamond Center for AIDS Research >NYC > While not common CD4 CD8 coexpression is seen and there is considerable published literature, see Prince, HE et al. Characterization of circulating CD4+ CD8+ lymphocytes in healthy individuals prompted by identification of a blood donor with a markedly elevated level of CD4+ CD8+ lymphocytes. 1994. Clin Diag Lab Immunol 1:597-605. Tom McHugh Department of Laboratory Medicine University of California at San Francisco
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:50:24 EST