Hi, friends: Would like to get your opinion on the detection of surface and intracytoplasmic Ig light and heavy chains. What antibody combinations are being used (e.g., kappa/lambda simulset with PerCP-CD19?) and what fixation/permeabilization method for the intracytoplasmic staining. Since weeks I am banging my head against the wall trying to get consistent data with various antibody combinations and fixation methods (I have used Caltag vs Pharmingen fix&Perm). Would appreciate any comments. Thanks, Elisabeth Paietta
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