Hi everybody, we have problems to detect beta-adrenoceptors on SF9 cells by flow cytometry. These cells were transfected and have about 500.000 to 1.000.000 beta-1-receptors. We get very nice figures with the microscope, but whenever we tried to detect the receptors by flow cytometry, we failed. We make a live/dead staining with PI, take the live cells and get only a 5 fold increase over the autofluorescence with 1 million receptors! Does anybody has experience with these cells? As we allways have nice pictures with the fluorescence microscope, it is not clear, why we should not see it by flow cytometry. Thanks for any help Andreas ------------------------------------------ Andreas Simm Physiologische Chemie II Biozentrum, Am Hubland D-97074 Wuerzburg FAX: +49-931-888-4113 Tel: +49-931-888-4128 E-Mail: Simm@biozentrum.uni-wuerzburg.de ----------------------------------------
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:50:23 EST