First a "thank you" to all of you who set me straight regarding the intimidating K-S test. Several mentioned that B-D software provides this test--do any other packages offer it? I'll have to check it out, even though that student didn't seem interested in wading in that deeply. Now, to David, You certainly have my sympathies. I had a user who was doing the same sort of experiments a few years ago, and we never produced any data I believed in. We saw exactly the same sort of variation, but the client never understood that this was a problem. In fact they wanted me to change the HV for each sample so that they lined up properly! Sometimes ya just wanna smack em.... Do you have a copy of Methods in Cell Biology v42, PartB ? The chapter on yeast by Dien, Peterson and Srienc has some very clear protocols, and the data presented look much nicer than anything we got. I never got a clear idea of how my client prepared their samples, but if I'm ever faced with yeast again I'll make them start with this chapter. I'll be happy to send you a copy if you (or your library) doesn't have the book. Steve --------------------------------------------------------------- Steve G. Hilliard steve@habanero.cb.uga.edu University of Georgia Cell Analysis Facility http://floweb.cb.uga.edu/Floweb/ --------------------------------------------------------------- On Thu, 20 Nov 1997, David McFarland wrote: > > I have a client that is staining yeast with PI and then doing cell cycle > analysis. From what I understand, the wild type yeast normally spend most of ----snip---------
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