RE: Quantitataive measurement of cell surface molecules

From: Bob Ashcroft (cytomat@netcore.com.au)
Date: Fri Nov 21 1997 - 04:09:24 EST


Listen up dEAR alICE,
Ken Davis talked about them at the Australasian FCM meeting in Auckland NZ 
last month.

One problem with his BRITE beads was that he said they had found that Abe's 
beads were under- (or was it over-  KeN??) estimating the MESF values it 
returned for FCMs, and by a factor of 10!! Either way, it looks like there 
is some more controversy ahead of us.

Cheers, Bob

-----Original Message-----
From:	Alice L. Givan [SMTP:Alice.L.Givan@dartmouth.edu]
Sent:	Friday, November 21, 1997 5:44 AM
To:	Cytometry Mailing List
Subject:	RE: Quantitataive measurement of cell surface molecules


While we are on this subject again, I agree that Philippe Poncelet has been 
a
good source of common sense on the issue of quantitation.  In addition,  BD 
has
started to sell some new beads (QuantiBRITE) that seem as if they may also 
help
in fluorescence quantitation.  I haven't tried them yet but would be 
interested
in comments from people who have.  These beads were,  to my knowledge, 
 first
described at the Rimini ISAC meeting in a poster by Ken Davis et al.

BTW,  are these companies all competing to put upper case letters in random
places throughout words?  Is this some sort of macho thing that has passed 
me
by?

aliCE

Alice L. Givan
Englert Cell Analysis Laboratory
Dartmouth Medical School
Lebanon,  New Hampshire
NH 03756 USA
tel 603-650-7661
fax 603-650-6130
e-mail givan@dartmouth.edu



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