To experts in flow DNA analysis, especially with paraffin embedded tissue: I am giving the medimachine (DAKO) a trial, and it seems to greatly enhance the mechanical agitation of the tissue.My question is, would using the medimach. over scissor mincing alow me to shorten my DNA procedure. The procedure,for paraffin embedded tissue is as follows (briefly): Run paraffin specimen down to water; mince tissue (medimachine) and add pepsin; incubate at 37 for 1 hour;wash twice in HBSS; add PI; incubate 10 min. at 37; run on flow. Thanks for any help. Sandy
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:50:21 EST