Dear D.R., Although I cannot furnish you with a reference, I recall reading some literature in the past which described the amount of DNA in human cells in mass terms was 7.5-7.9 picograms. Activated lymphocytes or proliferative cells (ie, G1 as opposed to G0 cells) within tissues were said to contain ~8.3 pg per nucleus - which may explain the near diploid peak seen by some investigators in the analysis of PB and some solid tissues. Trout red blood cells used for control purposes contain ~5.0 pg/nucleus whilst the DNA mass in chick red blood cells escapes me. I have remembered these figures for my research interests in cell ploidy and proliferation, but would not know where or if I have the source of this information now. If someone does give you a reference I would be pleased to know of it. Regards, Jeanette. At 07:22 PM 4/11/97 +0000, you wrote: > >Dear all > >Another question > >In a survey of a cultivated plant for local species/type/variant diversity, we >intend to use normal human lymphocytes as a reference standard for DNA >nuclear content. > >What we need to know is; please can anyone tell us the DNA content of >normal diploid human lymphocytes in mass terms per cell? > >Alternatively does anyone have any ideas for a reference text where we may >find this information? > >Failing that are there any better/cheaper/more appropriate standards which we >could use? > >Thanks. > >DR Lloyd >University of Birmingham >England > > >Regards > >D > >t; +44 (0)121 414 5264 >f; +44 (0)121 414 5324 >e; D.R.Lloyd@bham.ac.uk > > _______________________________________________________________ Jeanette Drew Department of Obstetrics & Gynaecology University of Western Australia / King Edward Memorial Hospital SUBIACO WA 6009 AUSTRALIA Tel: 61-8-9340-2768 ,-_|\ Pager: 61-8-9485-6570 / \ Fax: 61-8-9340-2636 JD -> *_,-._/ Email: jdrew@cyllene.uwa.edu.au v _______________________________________________________________
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