I've had intermittent luck running CyChrome labeled antibodies on our Calibur
with simultaneous detection of a second marker in FL4 (APC label). The major
problem is that when we do a single stain with the CyChrome antibody (e.g.
53-6.7, the anti-muCD8), there is a large spread of signal in FL4 that is
impossible to compensate because of the spread. The first thought was that
this is a time delay problem, but it doesn't get better if we run FACSComp
immediately before running the experiment. I still won't rule out a time delay
problem, but I thought I'd pick your brains to see if this was a common
problem with a robust solution.
Thanks,
John
--
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John Altman
Emory University
Department of Microbiology
and Immunology Office: (404) 727-5981
3119 Rollins Research Center FAX: (404) 727-3659
1510 Clifton Road Lab: (404) 727-8535
Atlanta, GA 30322 altman@microbio.emory.edu
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