Jet Velocity

From: Vincent Falco (vincent.falco@es.nemc.org)
Date: Thu Nov 06 1997 - 07:57:56 EST

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To All:
Way back when I was taught that the jet velocity out of a nozzle for sorting 
was optimal at 10m/s.This is a jet in air system,no quartz flow cell.The 
method to calculate this jet velocity from volume equations for a cylinder 
are given in Howard Shapiros Practical Flow Cytometry.While taking these 
measurements may be crude,the overall approximation is good.What I would like 
to know is,Does anyone check the velocity out of the nozzle?If so, what do 
you set your sheath pressure at to get this velocity?What is the velocity you 
use for your system,either BD, COULTER, CYTOMATION, OR HOMEGROWN. I am 
talking the standard type sorters.Not your TSO from BD OR OTHER HIGH SPEED 
SYSTEM.

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