>From owner-cyto-sendout@flowcyt.cyto.purdue.edu Mon Nov 3 15:44:54 1997 >Received: by flowcyt.cyto.purdue.edu (940816.SGI.8.6.9/930416.SGI.AUTO) > for cyto-sendout id QAA09580; Mon, 3 Nov 1997 16:53:59 -0500 >Received: from sorter.dfci.harvard.edu by flowcyt.cyto.purdue.edu via SMTP (940816.SGI.8.6.9/930416.SGI.AUTO) > for <cytometry@flowcyt.cyto.purdue.edu> id PAA08642; Mon, 3 Nov 1997 15:08:41 -0500 >From: HANDLEY@sorter.dfci.harvard.edu >Date: Mon, 3 Nov 1997 16:12:36 -0500 (EST) >Subject: Sorting into plates with CellQuest >To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu> >Message-ID: <878591556.840000.HANDLEY@sorter.dfci.harvard.edu> >Mail-System-Version: <MultiNet-MM(378)+TOPSLIB(158)@sorter.dfci.harvard.edu> > > >Hi Everyone! > >We are considering upgrading to CellQuest on our Vantage. Right now we >use the HP ACDU program, which isn't the most friendly program, but we >know it works and the limitations. How does CellQuest deal with sorting >into plates? And how well does it work? Could you use it to sort into >plates using FL4A and FL4W for HO cell cycle? We are interested in hearing >any pros and cons before we spend the money for the upgrade. > >Thanks, >Maris > >*************************************************************************** >Maris A. Handley Phone: (617)632-3139 (voice mail) >Dana-Farber Cancer Institute (617)632-3179 (laboratory) >J319A, 44 Binney St. >Boston, MA 02115 E-mail: handley@sorter.dfci.harvard.edu >*************************************************************************** > Buy the upgrade. All you do is define the gates in CellQuest, launch CloneCyt, tell how many cells you want sorted into the wells, and push start. That is all there is too it. I think it is a good program. I have had trouble with the ACDU unit itself, but the program is good. As far as sorting on FL4 area and width, I have never done it, but if you can define the sort gates, you *should* be able to sort on them. Good luck. Bill Nostrom Trudeau Institute Saranac Lake, NY ______________________________________________________ Get Your Private, Free Email at http://www.hotmail.com
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