I seem to remember a comment on the mail list regarding the induction of Annexin V positivity by manipulation of the cells of interest. Specifically, I remember the issue of freeze/thaw of banked cells leading to Annexin V positivity. Are there other cell manipulations that can lead to the same results? The reason I ask is that I am wondering if trypsinization of cells from culture plates will lead to Annexin V positivity like the freeze/thaw treatment? If so, it could answer some questions for us regarding some data we are fussing over. Any help or comments are enthusiastically welcomed. Haywood Pyle King Faisal Specialist Hospital and Research Center MBC #3, Box 3354, Riyadh 11211 Saudi Arabia e:pyle@kfshrc.edu.sa tel/fax: 966-1-464-7272 ext 32860
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