Hello group! This is a basic science question. Does anyone have a definition of oncosis which differentiates this method of cell death from apoptosis, including morphological and/or biochemical hallmarks? I have been treating HT29 (human colon adenocarcinoma) cells with a potential antitumor drug and I don't think this agent is killing the cells by apoptosis. Others in my group do not agree. The cells start to swell after 24-48 hours of treatment and by 96 hours the most of the culture is dead. At no point in the time course do the cells demonstrate true positive TUNEL results. I have only looked at DNA laddering once and got ambiguous results, i.e., I think I looked at too late of a time point. Any and all comments/suggestions would be appreciated before I repeat any more experiments. Thanks! Lisa Adams Pharmacia & Upjohn Kalamazoo, MI LAADAMS@am.pnu.com
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:50:17 EST